VEGF 121/VEGFA (AA 207-327) (Active) 蛋白
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for VEGF 121/VEGFA (AA 207-327) (Active) 蛋白 (ABIN667096)
抗原
VEGF 121/VEGFA
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Activity Assay (AcA), SDS-PAGE (SDS)
纯度
> 95 %
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产品特性
- AA 207-327
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序列
- MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR
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产品特性
- VEGF121, 207-327aa, Human, Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤4ng/ml.
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VEGF 121/VEGFA (AA 207-327) (Active) protein (His tag)
宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % AcA, SDS Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- VEGF 121/VEGFA
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背景
- Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
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分子量
- 14.2 kDa (122aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_001020541
抗原
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